LOCUS EY192914 314 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa87a02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY192914 VERSION EY192914.1 GI:159497400 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 314) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 779 was extended to value 780 (,) putative full insert read: vector-to-vector length is 357 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 506 PolyA tail found inside read...SR being truncated to 464 Potential full insert read...vec-to-vec length: 386 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..314 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa ccaaaaattg gcgatggggc gctgggagag aacaaacatt gaacgtgcgg 61 aaccatttgc tcttaaaaga aactctctaa cgcttttgta cattttttct ttgtcgacga 121 tgccatacag gcggattctg taggggccat atttctctac ttgattgtgt gttttcgaat 181 tagctctaac ttataacgca ttttcttcgt atgttcagct tcagagtcac cacttaatct 241 attcatttgc ccgctttcga tggatggtct cgtttcatcg ctttactaac ttcgaataaa 301 tgtatgattt atga //