LOCUS       EY192901                 325 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa86g10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to pir|D88449 protein F54D8.3 [imported] - Caenorhabditis
            elegans, mRNA sequence.
ACCESSION   EY192901
VERSION     EY192901.1  GI:159497354
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 816 was extended to value 816 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 519
            PolyA tail found inside read...SR being truncated to 475
            Potential full insert read...vec-to-vec length: 397
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac ccgcgacctg gacaaggcgc tctacgtggc gaacaacatc cgcgcaggca
       61 ctgtctgggt gaactgctac gacgtgttcg acgcggcggc gccgtttggc ggctacaaaa
      121 tgtccgggat cgggcgcgag ctgggcgagt atggactgtc tgcgtacaca gaggtgaaga
      181 ccgtgaccgt caaagtgccg caaaagaatt cgtgaatctg agaccagggt agagatcaat
      241 gaacaaaaac acattgactg ttctgattcc gagcattttc cacaaatatt gtaatacgaa
      301 ctgtcaaaac attcacctca atata
//