LOCUS EY192894 618 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa86g02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY192894 VERSION EY192894.1 GI:159497347 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 618) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 791 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..618 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcgg atggctgcgc ggtattttgg tgcaaccgat tggaggttct gcgctcgcgc 61 catgtccact acaatctctc ggtgcaatca atggacaagg acaattgcgg tcaaattttg 121 ttgtttgggc tggtcggcac gggcacacgg atttgcgttt cgaacacaca catttactac 181 aacatgaaga ggggcgaagt gaaactcgcc cagatggcct atctgctcgc ctctatgcac 241 cagatggaat cagaattcag cgcagaatat tcttctcagt caacacagaa caaagaaatg 301 tccaatctga actacggcca tttcgcgggt cacattctgt gcggagattt caacatggag 361 cctgctagtc ctttgcacag attcgtgctc aatggcactg tcaatttggg ggccaatacg 421 cgttttgaaa tggcgcacga attgcttcga gatcaacagc agcagcagcc attctgttcg 481 ttcactcccg cactcccact acccgccaat aaaatgcgca tctccgcccg ttgcatcttc 541 gtggaggact cgtcccaagt gctggacatc cacaaggacc agctccggca tccgttcagc 601 ttcgcctcgg tctatgag //