LOCUS       EY192888                 327 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa86f08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192888
VERSION     EY192888.1  GI:159497313
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 831 was extended to value 832 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 521
            PolyA tail found inside read...SR being truncated to 480
            Potential full insert read...vec-to-vec length: 399
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggag ccgctacact ctcgtgtact tcaggcacgc gacggcatcc aacacaagat
       61 tgactctctg caagtggagg cgcacgactt tgtggagtcg ctgatcgagc aggccagagc
      121 gttgcgccca agtggtggcg cagtggagca ggcggacacc aagaagacgc ggctggccgc
      181 tgcccacatc atcaaacagt tgaatgcgct cagcgccgag gcgcgtgcca atttgggacg
      241 cgtgttcccc gaaattgtga ccggcatcga gagcacccaa ttccaggcgt tggccaatgg
      301 acagagcgaa ttgtgagcag cggcgga
//