LOCUS       EY192887                 310 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa86f06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192887
VERSION     EY192887.1  GI:159497312
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 814 was extended to value 814 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 504
            PolyA tail found inside read...SR being truncated to 461
            Potential full insert read...vec-to-vec length: 382
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga gcaatgcttc aacgcgtgtg tagtggactt tacaggcagg aatatcaacg
       61 agaaggagga gcggtgttcc aagaattgta tggacaaatt ccttaaggcc ggacagaggt
      121 tgtcgatgcg tttccaggag tatcagttgc tcaacatgga ggcccaaggg gcgcctgttg
      181 cggccaccaa gaacgcgcaa ccgggaacgt gatactaggc atatgctagc gcccacacta
      241 cttgttaact tttcttttgc tcaatttttg aatagtccga acagcacaag taaagttgtt
      301 aaatagaaca
//