LOCUS EY192883 261 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa86f01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY192883 VERSION EY192883.1 GI:159497308 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 261) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 796 was extended to value 797 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 457 PolyA tail found inside read...SR being truncated to 412 Potential full insert read...vec-to-vec length: 333 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..261 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtg catcaaatta tccgatcaaa agcgccatac ccctccactt ttattatttt 61 gctacgttcc ttgatatgtg ttatcggtat caacagcctc agccagatga ttgttatttc 121 tgcctctagg ctactatttt aataatgctg ttcgtgtttc tggaaatttc ccacattatc 181 tcaatttgta ttcgcctaat agtgtttcgc atttcctaaa atcaaaacca tttccggttg 241 cttaaagttc ttatcccctt a //