LOCUS       EY192877                 494 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa86e07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192877
VERSION     EY192877.1  GI:159497273
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 816 was extended to value 817 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 147
            original value before phred recall for SR was 682
            PolyA tail found inside read...SR being truncated to 645
            Potential full insert read...vec-to-vec length: 566
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat tgatgcggca ggaaccggtt atggacaaca ggcgagccgg cagattggaa
       61 tcctcctgaa gctactccag ctggatcatg gttattggac gtcgttttac aacgctccga
      121 ctcttgacca gcagcaatgg ccactgactc acacgtcgag acgaaacgcc gcaaccggtc
      181 aaagacactc gccagctgtc actcagagca gccaggccat ggattcgaac aaccccgtgt
      241 gcaccgaaaa caagcagggt ctggtgcagc gaatggtcta caaggaacct cacttcggaa
      301 aactgtatta gcagggatgc aatccacttc aataatcctt tgtctggctt acctcttctc
      361 cattccagat ctgactaatc cttatcccta ttaatttgtg tattactcat ggcctggctt
      421 gatttttgtc atggaaccac aattctcaac tctttatttg tgttacactg tatcgataaa
      481 atttatttaa gcga
//