LOCUS       EY192868                 522 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa86d09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192868
VERSION     EY192868.1  GI:159497233
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 714
            PolyA tail found inside read...SR being truncated to 673
            Potential full insert read...vec-to-vec length: 594
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggaca atttttgaaa aagaattatt ttttgataaa atgctcttgc tttcacctaa
       61 atgtgtcatt ttttgttcat aatgttcata aatagaatcg tgatcgactg ccgaccagca
      121 aaaatgaaca agtgccccaa atgagcaggg cggagaggca cttgctggtc caggcggtca
      181 tcctctttag cgggctgttg gccctcgact acgggtggcc ctcgttccgg ctgggccagc
      241 aaatctacat tttcgccgca ctcctctact gcggcctcaa cccagtgctc tatctggcaa
      301 tgggcactga attccgacaa cgcttttgcg cacgtaccga tggacctgca tggagatgga
      361 aaagtcagtt gtgcttgaga tggacactca ttggctcccg ccagataaaa ataccagtgg
      421 atgcactact ccattcgagt tgggccgaag ccaagagaaa accttgtaaa ttaacgacac
      481 cacaattcgt ttttaatttg aaatttgaat cataaaataa ca
//