LOCUS EY192865 111 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa86d05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY192865 VERSION EY192865.1 GI:159497230 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 111) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 864 was extended to value 865 (,) putative full insert read: vector-to-vector length is 205 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 353 PolyA tail found inside read...SR being truncated to 262 Potential full insert read...vec-to-vec length: 183 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..111 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggat caatcgtaaa caccagatga caatggcata ttatttataa aatttgtttc 61 ctatgttctc ctccaattag taaatatact tttttaactt ttgcaaaaat a //