LOCUS       EY192850                 369 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa86c02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to gb|AAO43307.1| putative polyubiquitin [Arabidopsis
            thaliana], mRNA sequence.
ACCESSION   EY192850
VERSION     EY192850.1  GI:159497156
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 563
            PolyA tail found inside read...SR being truncated to 520
            Potential full insert read...vec-to-vec length: 441
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggtc aaggccaaga tccaggacaa ggaaggcatt ccgccggacc agcagcgtct
       61 gatcttcgcc ggaaagcagc tcgaggacgg tcgcaccctt gcggactaca acatccagaa
      121 ggaatccacc ctccatctgg tgttgcgtct tcgcggtggt tccgcctgag tcagagaacg
      181 cgcagaggaa tatggcgtct tgagatgtgg accaatttta attcctgttc tgatcacaca
      241 aaacacaatc cacatgtttt acgatataca taacagcaga aaacaaccta cattcattaa
      301 gtaatcatgc tttctcgata tgtaattctg aaatggcttt cgcggaacag taaagaatac
      361 aaaatgaca
//