LOCUS       EY192849                 349 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa86b12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192849
VERSION     EY192849.1  GI:159497155
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 724 was extended to value 724 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 585
            PolyA tail found inside read...SR being truncated to 500
            Potential full insert read...vec-to-vec length: 421
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggag gagacattca acgccgtcaa catagagagg atcgagggac tttcgcgtga
       61 agagataagc cgggagctcc tcaacagcga gaaggacaat cgacatttgc agcaacgtgt
      121 catcagcctg aaagaaagtc acgagcagtt ggtccttgag aatatgcagt taaaagctgt
      181 gcttgcagag aacgggatcg aggtgaatac aacgactgat gacaagaaat gaggagacaa
      241 atgatgctat accaccgatc aatttcattt tcctaccatt gcggaatata catcatcatt
      301 tacaaattta gtgccacctt aggcgcattt tcaaataaat aaatatgca
//