LOCUS       EY192845                 442 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa86b05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192845
VERSION     EY192845.1  GI:159497151
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 823 was extended to value 823 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 147
            original value before phred recall for SR was 632
            PolyA tail found inside read...SR being truncated to 592
            Potential full insert read...vec-to-vec length: 560
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcaa ggatgaagac atcactatcg actttcaaga tttgcagcca atgcagccgc
       61 agtgggtgat caggccagag tggttgccgg accagacagg gacgccaccg gctcaatgct
      121 gtcgattgag aatcaggagg gcgttttgca gggctctcgc ggggtgaccc gctggcaatt
      181 ctctgcaagt tctgtttaaa cgctcgactc tgcacacaat ttctcaaggg cgttcacaaa
      241 gctgaacggg gcaaacggct ttccacagcc tgggaacaca gcacagggca atgctgagca
      301 acactgtttc caccagtgcc tggatgagaa cgattatttc acaagatgat ctgggatcga
      361 actcgcctgg ttcgggtacc acaacgagag atggcaattt gggtactctt gcccagtgct
      421 cggatataaa tggtacataa ga
//