LOCUS       EY192831                 462 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa86a03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192831
VERSION     EY192831.1  GI:159497517
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 825 was extended to value 825 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 654
            PolyA tail found inside read...SR being truncated to 613
            Potential full insert read...vec-to-vec length: 534
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg ccaggacgag gcgcttatcg atgcgatcaa tgcgcacccg acggcggagg
       61 atcaatttct gttcgcactc cccgtttgcg cgccttacac ggccatgcaa aagttcaaat
      121 tcaaggtgaa agtaacaccg ggcacattca agcgcggcaa agctgtgaag caggccctgg
      181 agctgttcaa tcgtgacaag tccctgacgc tggcggagag gaccctgctc aaggggctga
      241 gcggcgacga tcggccgaca gcgctgttgc cgagcaaagt tcgcatatcc gcacctgcca
      301 tgaatctggt caagaagcgc aaataaaaat ggacacatga ttgttactat aatacatatt
      361 attgctgttc atattctgct accctgtgaa aaagagaaaa agactgtcca tagctccacc
      421 taactattaa gttttaactc agatgtcacg ccatactgtt ta
//