LOCUS EY192830 226 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa85h12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY192830 VERSION EY192830.1 GI:159497516 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 226) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 458 PolyA tail found inside read...SR being truncated to 377 Potential full insert read...vec-to-vec length: 298 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..226 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtac tgacgcgagc cttcaacgca ttacacgacc aactgatgaa gtatggtttt 61 atctgctcag acagctactc ctggattatt gagcacggcc tcatggatcc aaaaatgaag 121 tttgaagcgg caaatcagta aaagaaaaaa tctgattgca gcgatgaaaa tgaatactaa 181 ttttcatttt tatttgactt ccaatgtcgt ttaaaattaa aagtta //