LOCUS       EY192823                 583 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa85h04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to sp|P27443|MAOM_ASCSU NAD-dependent malic enzyme,
            mitochondrial precursor (NAD-ME), mRNA sequence.
ACCESSION   EY192823
VERSION     EY192823.1  GI:159497509
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 831
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcac taagaagccc atgtccgaag caaaatacat atttttgggc gctggcgggg
       61 ctgctctcgg tgttgcagaa atgtgtgttc gtcaaatgat cagcgagggt atcagccaag
      121 atgaggcgtg tgcgcgtatt ttcatgatgg acatcgacgg gctgatcacc cggtcgcgta
      181 gcaacatgga cccgcgccac gtgccattcg ccaaggacat gcacgagact aaggatttgc
      241 tggaagttgt gaacaacgtg ggccccgatg cattgattgg cgcctcgacc gtgaggggag
      301 cgttcaccca cggcatcatc cgcaaaatgg ccgagttcaa ccagcgcccg atcatctttg
      361 cactctccaa tccgaccagc aaagccgagt gcacggcgga ggacgcctat cgactcacaa
      421 atggaactgt gcttttcgca tcgggcagcc ccttcaagaa cgtggaactg cacggtcgcg
      481 tcttcaaacc gggccagggc aacaacgcgt acatattccc gggcgtggcg ctggccgtca
      541 tcctcttaca agtgtccacc atcgacaaca agctgtttct act
//