LOCUS       EY192822                 424 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa85h03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_187574.1| 60S ribosomal protein L4/L1 (RPL4A)
            [Arabidopsis thaliana] sp|Q9SF40|RL4B_ARATH 60S ribosomal protein
            L4-2 (L1) gb|AAF23293.1|AC016661_18 putative 60S ribosomal protein
            L1 [Arabidopsis thaliana] gb|AAL09727.1| AT3g09630/F11F8_22 [, mRNA
            sequence.
ACCESSION   EY192822
VERSION     EY192822.1  GI:159497508
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 617
            PolyA tail found inside read...SR being truncated to 575
            Potential full insert read...vec-to-vec length: 496
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggtg tggaccgaga gtgctttccg caaattggat tctatcttcg gcagctttgc
       61 caggaaatct gatgttaaga aggggttcac tctgccccgc gcaaaaatga ccaactccga
      121 cctcgtgcgt atcattcgct cggacgaaat tcagaaggtt gtgcgcccga tcaagaagca
      181 gaggaaggtc gcaaaggttc accgcaatcc gctgaagaag caccctttga tggtcaaact
      241 gaacccctat gcttccgttc aacgcagagc cgcaattatc gcgtcggcta aggggcaaca
      301 gcaaaaggcc tagaagaatg aattgagtgg gtaccggaca aacatcagct ggcggaaaat
      361 tcgaatgttg accatttttg ttggcaaaat tgttatttca tgttgcaaat aaactgcacg
      421 ggta
//