LOCUS       EY192819                 306 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa85g12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192819
VERSION     EY192819.1  GI:159497505
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 789 was extended to value 789 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 530
            PolyA tail found inside read...SR being truncated to 456
            Potential full insert read...vec-to-vec length: 378
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggacg atgcggtgcc tttcgtcctg atggatcgga caatgaacgg catatccccc
       61 gcgtatgttc ccatcacttg ctgagctggt ccaatcgtgc cctctctgct tggatgtact
      121 tgtcgattac gtcgctagtg gttattgctt cactggacaa aagattttcc agctcgacca
      181 tcgctgtgaa cacatctgcg cggttaagag gcaaaaagaa acatagcagc aggataattg
      241 agatgcacag tgacatccta aaaaggaaaa acgggaagtg attttggtat aaaaacgatc
      301 aatata
//