LOCUS EY192817 445 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa85g10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|XP_130165.1| folylpolyglutamyl synthetase [Mus musculus], mRNA sequence. ACCESSION EY192817 VERSION EY192817.1 GI:159497503 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 445) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 814 was extended to value 815 (,) This trace has been recalled with phred original value before phred recall for SL was 154 original value before phred recall for SR was 818 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..445 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggatt gaagagcagg tggatgtcgc agttgtggag gtggggatcg gcggcgaatt 61 cgattgcacc aacgcgattc agcgtccaat tgtctgcgca ataagcacgc tggacatcga 121 ccacacaaag ttgctgggcc aatcgctgga ggagatcgcc tggcataagg cgggtatcgc 181 taaaaaggga gcggtgctct tgagtgtaga acacccggaa aaggcaatgc gcgtggtcgc 241 ggagagatgc gccgagaaag agtgtccact ctttgtcatt ccgcccctcg cttcctacca 301 actttctcca ccccatcggt cactccatct gggcattgct ggacccccgc aacacataaa 361 tgcatcactg gcactgcaat cagcacgttt ctggctctgg cgtgtccaca aacgacacat 421 tgaattgaat cccactcgta ccgat //