LOCUS       EY192810                 404 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa85g03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_500630.1| Ribosomal Protein, Large subunit RPL-20
            (21.0 kD) (rpl-20Co) [Caenorhabditis elegans] sp|O44480|RL1X_CAEEL
            60S ribosomal protein L18a gb|AAB92041.2| Ribosomal protein, large
            subunit protein 20 [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY192810
VERSION     EY192810.1  GI:159497496
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 832 was extended to value 832 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 599
            PolyA tail found inside read...SR being truncated to 555
            Potential full insert read...vec-to-vec length: 476
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc tctgttaaaa actttggcat ctggcttcgt tatgactctc gaaccggcca
       61 ccacaacatg tatcgcgagt accgcgatgt tacggaggca ggtgcggtaa ctcagtgtta
      121 tcgtgatatg ggtgctcgtc atcgtgcaca agcagaccgc atccaaatta tcaaggtcca
      181 gacaatcaaa gtggaggact gcaagcgtgc tggtgtgaag caattccaca actcgaagat
      241 ccggttcccg ttgccgcatc gcgtgaatgc caaacggcgc aagatggctc cattcaccac
      301 tcagcgtccg aagacccact ttgcttgaat tggcaatcgt tacacattgt tgctggaccg
      361 tatttgtttc atttcaatat ggttgaaagt tatgaattgt tata
//