LOCUS       EY192801                 449 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa85f03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_505643.1| LYSozyme LYS-2 (30.3 kD) (lys-2)
            [Caenorhabditis elegans] pir|T26555 hypothetical protein Y22F5A.5 -
            Caenorhabditis elegans emb|CAA16324.1| C. elegans LYS-2 protein
            (corresponding sequence Y22F5A.5) [Caenorhabditis elegans], mRNA
            sequence.
ACCESSION   EY192801
VERSION     EY192801.1  GI:159497487
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 643
            PolyA tail found inside read...SR being truncated to 600
            Potential full insert read...vec-to-vec length: 521
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcac cgatgacaac cgtggagtca atgcgcccct ttggtactgg catacgcttg
       61 gccgcggctc tggagccaaa acctacaaag acatgagcga cttccgagca ttcggctcat
      121 ggcaaagtgc gtcgccgaac ctgaaagaat atgatgttgt ggacggaagt atttgcggaa
      181 aataccttag ttggagcatt ttcacaacaa accgggcgat ggttgttggc aaaaatgaga
      241 ataacatgac actgaagaag ctgctgaacc caaaacaaga aaacgccgaa atgattgggg
      301 gcacattttt gcagcagtga aggaaattgg aaaaaggaat tttataaatg gataaaaata
      361 taagatgtat agtattcact acctacatat ttgatatttt caatccgatt gagttattag
      421 tttcgttact aaaaataaaa atcagtgga
//