LOCUS EY192800 438 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa85f02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_491282.1| Calponin homology (CH) domain containing protein family member [Caenorhabditis elegans] pir|T29467 hypothetical protein F28H1.2 - Caenorhabditis elegans gb|AAB52338.1| Calponin protein 3 [Caenorhabditis elegans], mRNA sequence. ACCESSION EY192800 VERSION EY192800.1 GI:159497486 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 438) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 632 PolyA tail found inside read...SR being truncated to 589 Potential full insert read...vec-to-vec length: 510 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..438 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggggg attcaggcgg gctccgtcaa gaagatccag cagccgactg gcaacttcgc 61 ctgcatggag aacatcaacg ctttcgtgtc gttcgcgaag agccagggag tgcccaccga 121 ggagaccttc cagagcgtgg acttgttcga ggcacgtgac ctctactccg tgtgcatgac 181 cctgttgtcg ttgggccgaa ttatggagaa gaagggaaag ccgaacccat tctctggatg 241 aagagtagaa gtgagtgcag caaaagacgg acagagcatg tgctatcgct cccattcgag 301 aactccccgt tttgcgaatt ttctccccgt gtgcgacctt gcaacaatac cgaggcgtta 361 actgttttcc cctctctctc ctctcaaact gtgggcattt gaaaatagcg atgccggaat 421 aaatggccaa ttcaaaca //