LOCUS       EY192784                 499 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa85d09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_506478.1| ATP-dependent helicase [Caenorhabditis
            elegans] pir|T22917 probable ATP-dependent RNA helicase F58E10.3
            [similarity] - Caenorhabditis elegans emb|CAB04518.1| Hypothetical
            protein F58E10.3 [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY192784
VERSION     EY192784.1  GI:159497470
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 690
            PolyA tail found inside read...SR being truncated to 649
            Potential full insert read...vec-to-vec length: 571
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcaa taggactctg gtgttcgtgc aaactaaacg agcggctgac gatttgactc
       61 gtgacatgcg aaggaaaggt tatcctgctt tttgcatcca cggcgacaaa tcgcagaccg
      121 agagagattg ggtcatgcgc gaattccgag agggcaatac accgattctg cttgctactg
      181 atgttgcagc gcggggactg gatgtatccg acatcaagta tgtgataaat tacgatttcc
      241 ctctggatgg cattgaaagt tacgtgcacc gaattggccg aaccggacga caggacaaga
      301 ggggtaccgc ctacacattc ttcactccca gtaacgcttc gctcgccggc gacctcatag
      361 gcatcctgga agaggcgaag caggaggttc cctcgcaact gcgagaattt gtgggcggta
      421 gcagcacaag caacaatcgc aatttccgcc gcttccgttg agattgctat ctatttagtt
      481 tagttataaa tcattgtta
//