LOCUS EY192780 248 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa85d03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY192780 VERSION EY192780.1 GI:159497466 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 248) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 147 original value before phred recall for SR was 440 PolyA tail found inside read...SR being truncated to 399 Potential full insert read...vec-to-vec length: 320 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..248 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcaa gagcgaaata atcaccttcc cattgattat caacaacgtc tgcgttttat 61 ggctctttcc aaacaggcaa aatatggaac attctgtgat gatgcggttg attgcggatg 121 gtttgacata gtcggaaatg atgcaaagtg ggttttgcgt gagaataaat ttaatatgta 181 aaacttcaaa aaaagctgaa aagttatcgg ctgaaagtca aagaagaatg caataaataa 241 ttttttta //