LOCUS       EY192774                 595 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa85c09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_508841.1| Actin ACT-4 (41.8 kD) (act-4)
            [Caenorhabditis elegans] sp|P10986|ACT4_CAEEL ACTIN 4 pir|S27135
            actin 4 - Caenorhabditis elegans emb|CAA34720.1| actin
            [Caenorhabditis elegans] gb|AAB04575.1| Actin protein 4, isoform a
            [Caenorhabd, mRNA sequence.
ACCESSION   EY192774
VERSION     EY192774.1  GI:159497460
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 812 was extended to value 812 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 815
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggcg tggttactcg ttcaccacca cggccgagcg tgagatcgtg cgcgacatca
       61 aggagaagct gtgctacgtg gcgctggact tcgagcagga gatggctacc gctgcctcct
      121 cctcctcgct ggagaagagc tacgagctgc ccgacggtca ggtgatcacc gtcggaaacg
      181 agcgcttccg ttgcccggag gccctcttcc agccgtcctt cttgggcatg gagtctgccg
      241 gcatccacga gacgtcgtac aactccatca tgaagtgcga catcgacatc cgtaaggacc
      301 tgtacgccaa caccgtgctc tctggcggca ccaccatgta cccgggaatt gccgaccgca
      361 tgcagaagga gatcaccgct ctggccccgt cgacgatgaa gatcaagatc atcgctcctc
      421 ctgagcgcaa gtactccgtc tggatcggag gctccatcct ggcctcgctg tccaccttcc
      481 agcagatgtg gatcagcaag caggagtacg acgagtcggg cccgtccatc gtgcaccgca
      541 agtgcttcta agcagggaca gcggacagcg agacagacac agaggaaggc agaca
//