LOCUS       EY192694                 309 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa78e10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to gb|AAK18279.1| major allergen [Brugia malayi], mRNA
            sequence.
ACCESSION   EY192694
VERSION     EY192694.1  GI:159498027
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 862 was extended to value 863 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 153
            original value before phred recall for SR was 508
            PolyA tail found inside read...SR being truncated to 461
            Potential full insert read...vec-to-vec length: 445
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga tcctattggc aggagaacca tcgcttcaac aagacctgcc gtccgaatgg
       61 cgcagtagag gtcgtcaact gtgtgtccaa ggacggatac caaatcccgc tgaacagcca
      121 agtgatccgc aacggggcaa aatacagctg cgagatgact ccccagggca tcatccgttt
      181 cactgcaggc cccgccgatg attgagtgaa tgaaaatggc tggtagaaga ggagaccatt
      241 ggcaaataca tttgaaacac tgttattttg ccgcaccatt tttagccatg gatcggctta
      301 ttttttaca
//