LOCUS       EY192649                 279 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa72f02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_502267.1| Dehydrogenase glutamate (58.8 kD)
            [Caenorhabditis elegans] pir|T28022 hypothetical protein ZK829.4 -
            Caenorhabditis elegans emb|CAA98074.1| Hypothetical protein ZK829.4
            [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY192649
VERSION     EY192649.1  GI:159497892
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 478
            PolyA tail found inside read...SR being truncated to 431
            Potential full insert read...vec-to-vec length: 417
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg cttctgagaa agacatagtg cactcgggtc tcgaatacac catgaaccgc
       61 tctgtgaagc aaatcatcgc taccgcggag aagtacaatc tcggcctgga tctgcgcaca
      121 gccgcatacg ctaacgccat cgagaaagtc taccagacat accagactag cggattcacg
      181 ttcacttaga ctcgtgcgct ataataggtt cccaccggtg atgaacccgt tttatttacc
      241 ttttccttct aacaaataaa ggcaagaaat cttgtttga
//