LOCUS       EY192574                 326 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa67f07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192574
VERSION     EY192574.1  GI:159496270
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 896 was extended to value 896 (,)
            putative full insert read: vector-to-vector length is 402 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 554
            PolyA tail found inside read...SR being truncated to 509
            Potential full insert read...vec-to-vec length: 325
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggagg cgaatatccg ttcatcgcgg aaattggtaa cggagatctg ctcgttgact
       61 ctgccgccat cgaccgggag ttggcttctc tttcttttcg ctgccgagca acaaatgagc
      121 tcggagttga ttctgcggac tctaccgtta ttttattgaa ggatgaacag tgaaggaata
      181 cgtacgcgca tgggacatat gcgtgaatga gaaataacat gcgaattgag attgatacaa
      241 agtaatgcca gaaccgaact gtacaataaa gaatggttga aaaaatcagt atacaacacc
      301 aaaaaattta tttttttaat ccaacc
//