LOCUS EY192558 389 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa67a02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_647979.1| CG10590-PA [Drosophila melanogaster] gb|AAL49023.1| RE48767p [Drosophila melanogaster] gb|AAF50762.2| CG10590-PA [Drosophila melanogaster], mRNA sequence. ACCESSION EY192558 VERSION EY192558.1 GI:159496229 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 389) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 748 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..389 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggag acatcaacgg cttttatcta cacgcacaag aaatttgaac ttggttacaa 61 tgggcaaaat attgtcgttg tggatgtggt tgcagaggaa aagcaagaaa ttcaaccaaa 121 tcaagaattg tctttcactt atgaagtgat ctggaagcaa tctgagatac tcttcgacaa 181 gcgttttgag cggtatctcg atcccagttt cttccaacat cgtattcatt ggttcagcat 241 tttcaattct ttcatgatgg tcattttttt ggtcggactt gtctggatga ttttgttgcg 301 aacgctatct attgattatg cccgatatca caaggagcaa gctgatgttg aagctgatga 361 aatggactta ccggatgatt acggatgga //