LOCUS       EY192553                 277 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa65h06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to sp|P41340|ACT3_LIMPO ACTIN 3 pir|S49480 actin 3 -
            Atlantic horseshoe crab emb|CAA86290.1| actin [Limulus polyphemus],
            mRNA sequence.
ACCESSION   EY192553
VERSION     EY192553.1  GI:159496197
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 153
            original value before phred recall for SR was 523
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggca actcgatcat gaagtgcgac attgacatcc gtaaggacct gtacgcgaac
       61 accgtgctct ccggcggcac caccatgtac ccgggaatcg ctgaccgcat gcagaaggag
      121 atcaccgcgc tggcgccttc caccatgaag atcaggatca tcgcgccgcc tgagcgcaag
      181 tactccgtct ggatcggtgg ctccatcctg gcctcgctct ctaccttcca gcagatgtgg
      241 atctcaaagc aggagtacga cgagtcgggc ccctcga
//