LOCUS       EY192524                 442 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa63g10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192524
VERSION     EY192524.1  GI:159497386
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 889 was extended to value 890 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 639
            PolyA tail found inside read...SR being truncated to 593
            Potential full insert read...vec-to-vec length: 570
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggagct tgagactgaa aatattttcc aatatgcgtt gcgcattcat cctgctcttg
       61 gtcgtcatcg gtgtgcttgc catgctggag ctcaccactg ccgatccaat cactcacttc
      121 aatgagcgcg tggtcgggct gctggtggag cacgaggcgg acgagccgat gattgagcgc
      181 atgaggcgct ggggcggcgg cggcatcgga gcggagcgaa tgcgacgcca aatgggcggt
      241 ggagccggtg gtggtggcgg aggcggcaga aactgttgcg actgctgcac tggctcttgc
      301 ggatgcgaga tttgctgtgt accgaactgg ggccgcaaat gaaggaacgg agattgggaa
      361 caataaacag tcgatcaata gacaaattga tatgccattt atgcggtgaa tggactgaaa
      421 ttgcgtacaa atttgaattc ca
//