LOCUS EY192503 227 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa62g11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY192503 VERSION EY192503.1 GI:159497307 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 227) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center putative full insert read: vector-to-vector length is 307 (,) This trace has been recalled with phred original value before phred recall for SL was 153 original value before phred recall for SR was 460 PolyA tail found inside read...SR being truncated to 381 Potential full insert read...vec-to-vec length: 364 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..227 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggggc tggattccgg gcaccatcca cgccttctgg tactgcttct tccgttgagc 61 ccaatccccc tctcttcttc aatactcctc tacccccggt tataagcatt ttgcgttccc 121 ttcctgcttc tagtagtgtg actttttggc tgttgcccac caccaaatcc ctccactcat 181 gaatttctca acgaccgata aatttataaa taaactgatt ccattta //