LOCUS       EY192481                 253 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa57g03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192481
VERSION     EY192481.1  GI:159497226
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 321 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 473
            PolyA tail found inside read...SR being truncated to 407
            Potential full insert read...vec-to-vec length: 384
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggcatg ggctgtgatc aattttaaat tgtcaatatc gaaaactgta ttttattttt
       61 ctgctccaaa accgtccata caccggccaa agcgctgtat tttcacaaat cacacgaccc
      121 ttttaaattc aattcttaaa ttaattgtat gattgaattc accaagtacg tttcaaccat
      181 atattttgaa cgaacggttc catatattta tcatcgtgaa gagcttgtcc gaataaatct
      241 tgaacaaaag tga
//