LOCUS       EY192477                 212 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa57f05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192477
VERSION     EY192477.1  GI:159497222
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 258 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 410
            PolyA tail found inside read...SR being truncated to 364
            Potential full insert read...vec-to-vec length: 351
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtgc tccccagtgg gcgggctctt tattgcgcag cagcaacgga tcgcactggc
       61 caatctgctg gggttgggcg gatgaaacgg aaaggaaacg gaactgctgc cgacccgaaa
      121 gtgatgcgat tggaatggct gccgattgtt tgtctgttgc ccaatgaata aataaggccc
      181 attgtcctgg aaagggagtg agccgaccgt ca
//