LOCUS       EY192472                 388 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa56h06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192472
VERSION     EY192472.1  GI:159497188
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 911 was extended to value 911 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 582
            PolyA tail found inside read...SR being truncated to 540
            Potential full insert read...vec-to-vec length: 527
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggatt cgacctgaac tcagacgtga atacccgctg aacttaagca tatcagtaag
       61 cggaggaaaa gaaactaaca aggattcccg tagtaacggc gagtgaactg ggaagagtcc
      121 agcgctgaat cttggtgcct ctggcgccaa gaggtgtagc gtacagactg cgagtttggg
      181 ctggcgcgtc agttcaagtc tcccttgaca ggggtccctc agaaggtgca aggcctgtcc
      241 aacgggtgca gacagtccat tttcgcgtgt cttggagtcg ggttgtttgg gatcgcagcc
      301 caaagaaggt ggtaaacttc atctaagact gaatatgact acgagaccga tagcgaacaa
      361 gtactgtgaa ggaaagtcga aaagcaca
//