LOCUS       EY192461                 453 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa56f10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_505818.1| Actin ACT-2 (act-2) [Caenorhabditis
            elegans] sp|P10984|ACT2_CAEEL Actin 2 pir|T24448 hypothetical
            protein T04C12.5 - Caenorhabditis elegans emb|CAB04675.1| C.
            elegans ACT-2 protein (corresponding sequence T04C12.5)
            [Caenorhabditi, mRNA sequence.
ACCESSION   EY192461
VERSION     EY192461.1  GI:159497148
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 951
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggta cgtggccatc caagccgtgc tctcgctcta tgcgtccgga cgtaccaccg
       61 gcattgtgct cgactctggc gatggtgtga cccacaccgt gcccatctac gagggttacg
      121 cgctgccgca cgccatcctc cgtctggact tggccagccg tgatctgacc gactacttga
      181 tgaagatcct cactgagcgc ggttactcgt tcaccaccac cgccgagcgt gagatcgtcc
      241 gcgacataaa ggagaagctc tgctacgtgg cacttgactt tgagcaggag atggccaccg
      301 ccgcctcctc ttcgtcgctg gagaagagct acgagttgcc cgacggacag gtgatcaccg
      361 tcggaaacga gcgcttccgt tgtcccgagg ccctcttcca gccctctttc ctgggcatgg
      421 agtcggccgg catccacgag acctcgtaca act
//