LOCUS       EY192459                 396 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa56f07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192459
VERSION     EY192459.1  GI:159497146
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 911 was extended to value 911 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 592
            PolyA tail found inside read...SR being truncated to 548
            Potential full insert read...vec-to-vec length: 534
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtgg acaaattgaa aatttgaaaa ataacccaaa acattgaatt agttttaatt
       61 tatgaattct aaattattga cattaatttc aaaatagtcc gtttgaaaat tttgtcgttt
      121 ctccacttcc aaaaattgcc catcattttc agagaatgga cgcggcttca cattgggccg
      181 catttggacg cgtgatgggc gccatgtggt gtcggtctca caagagtcgt tgagccgctc
      241 cagaactgag aagatttcgc ggctttaaat gtgcaattcg ggactgagaa tgagaagact
      301 ttgatgtgct cgttgtcatt aaatagaaat cccattattg tttgtgtact ttgtgttgaa
      361 catttactat tttgtgccaa tgagaagggc accaga
//