LOCUS       EY192445                 330 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa56d02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192445
VERSION     EY192445.1  GI:159497666
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 414 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 565
            PolyA tail found inside read...SR being truncated to 483
            Potential full insert read...vec-to-vec length: 448
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac gccatcctgg ctctgttttc caataatcct cagcggatca acattgttac
       61 ggctcgtgga cgtctcaaca cacttgtgac cgccgatttg atccgtttcg aggtgctcta
      121 caacaacctc caaactaatg gaattggagt gctgaccgtg ggtgagatgg gagagctctt
      181 ccacttcttc cgggcactcg aacctgcatt tgtcgggtca catatcttcc gcgagtttta
      241 cgtgatggct gcgttcaacg gcatccgtcg ttagagatcg ttattcagat caattttgtt
      301 aaatcttttg aataaagatc gttaacttca
//