LOCUS EY192425 170 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa55g03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY192425 VERSION EY192425.1 GI:159497634 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 170) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 853 was extended to value 854 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 364 PolyA tail found inside read...SR being truncated to 319 Potential full insert read...vec-to-vec length: 242 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..170 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggctt cttcgaggcg gaccagctgg aggtggaggg cgacctggag cagctgcaca 61 tggtcggcgt caaggacttc taggcgcggc gagaacagca aaaatgatgt ggaacttacc 121 atccattttg gattgtgtgg ttttttgtct ttgataaatt gtcgatctta //