LOCUS EY192423 242 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa55f10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY192423 VERSION EY192423.1 GI:159497632 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 242) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center putative full insert read: vector-to-vector length is 543 (,) original QR value of 955 was extended to value 956 (,) This trace has been recalled with phred original value before phred recall for SL was 153 original value before phred recall for SR was 696 PolyA tail found inside read...SR being truncated to 395 Potential full insert read...vec-to-vec length: 384 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..242 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggggg ggatttcaga tgtttgattc ggattttcgg attggggcat gcctgtgctt 61 ggctgcagga aaccgcgacc agaagaacga gcaacagagt gggcacaagc agccgaagca 121 gcatagacat ttttagtgag caaggacaaa cttttggtgt gaattctctc tagttctgaa 181 acaattagat gtatacctac atatggattt gggaaatctg aaataaaatt caactccttt 241 ca //