LOCUS EY192421 281 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa55f07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY192421 VERSION EY192421.1 GI:159497622 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 281) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 894 was extended to value 895 (,) This trace has been recalled with phred original value before phred recall for SL was 152 original value before phred recall for SR was 522 PolyA tail found inside read...SR being truncated to 434 Potential full insert read...vec-to-vec length: 422 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..281 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtg agtgcagcgg gcagtgccga aatgatggat ggatcttcag gagctgcgct 61 cgatgaggag attgttgttc cctggaaaaa gttgtaccgt ccgagaggtc ctccccgctt 121 tgggaaacgg gcactggtgc agatggacga cgagtctgtg ccggtggctt ggaggaaggg 181 aactgaatga tggaagagcg gcaggaaagg acgcatgcgc agattggtcc attttcaatc 241 gaactgacga atgttggtga taaatgataa tgtgtgggcg a //