LOCUS EY192390 328 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa52g03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to sp|Q90YP3|RS28_ICTPU 40S ribosomal protein S28 gb|AAK95213.1|AF402839_1 40S ribosomal protein S28 [Ictalurus punctatus], mRNA sequence. ACCESSION EY192390 VERSION EY192390.1 GI:159497566 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 328) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 895 was extended to value 896 (,) This trace has been recalled with phred original value before phred recall for SL was 147 original value before phred recall for SR was 519 PolyA tail found inside read...SR being truncated to 478 Potential full insert read...vec-to-vec length: 466 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..328 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcaa gcataatttt taaacacgca atctagcatc gcaaaagaat ggagaagccg 61 atcaagctgg ccaaggtcac ccgcgttctg ggccgaactg ggagccaggg ccaatgcaca 121 caagtgcgcg tcgaatttct ggatgacacc aatcgctcga tcatccgaaa cgttaagggc 181 ccggtgcgcg aaggcgacat tctcacgctg ttggaggcgg aacgtgaggc gcgacgtttg 241 cgctgaaaaa tgactgccca agcatgcttg ttgttgttat ttctatagta aatgttgcag 301 ttgcaagtta aataaaatat tttcttca //