LOCUS EY192382 446 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa52f01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY192382 VERSION EY192382.1 GI:159497549 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 446) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 821 was extended to value 821 (,) This trace has been recalled with phred original value before phred recall for SL was 153 original value before phred recall for SR was 830 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..446 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggaac tgcagaccta tttctccatt ccgagcgcca cttccaatgg caattttgag 61 agccaagagg gggatccgca ctcgccgctc agcgtgttca agacggttgc caagctccac 121 cagaattccg acttcatttt gggcgagttc gagcactcga agccggccga cattgacctg 181 tacgtggtga agcgctaccc agccggcagc ggcttctacg tgctggccgt gaactggaac 241 ggcgaacagc gctcgctgag caacggcaaa ttgcccgtgg agctgaagga ggcgaaactg 301 tcttcggtgg aagtgctcgc ctctgccggg gagccgaatg ccccattggg gaaggtggac 361 ctgagcgagg gcgccaaccc actagatgtg ggcgcctacg gagccattgt gctcgaagtg 421 cagtgaatgg gagaggggaa ttgtgt //