LOCUS       EY192374                 399 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa52d10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192374
VERSION     EY192374.1  GI:159497541
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 847 was extended to value 847 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 153
            original value before phred recall for SR was 597
            PolyA tail found inside read...SR being truncated to 551
            Potential full insert read...vec-to-vec length: 461
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc aataacccga ccgtggagga ctgctgcatc tactacatca acggattata
       61 cccatccaac gggggcagcc aaaccctcgc catgcgcaaa acgttcctcg cgacgccgtt
      121 cttcacctat tacacgcagg agactacagt gagcatccag atcgcgacta ttagcatctt
      181 ctgtggctgc aattgaggcg caagacagaa gagagtggcg tccaacggat tcctttctcg
      241 aggaagtcac cacgtgttga gccgcacctt ttctctccag cactttcctg tgcatagtcc
      301 taaaagaggc aagacatctc cctttgaccc aaaacgacag aacaccgccg ctttttgtag
      361 ttttcttaca ctttccttgt gaataaatgg tgcgtgaga
//