LOCUS       EY192291                 466 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa44f04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192291
VERSION     EY192291.1  GI:159496821
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 669 (,)
            original QR value of 909 was extended to value 910 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 153
            original value before phred recall for SR was 822
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga cagacgcatt cttcggaaag gaactgatcg aacgcccaaa atgcaactct
       61 ctatttccgt tgtttccctg gttctcttgg gcgcggttgc agccgtccaa tcggccccca
      121 cgggatttgt gctcccgaac caggcggaga tcgagagcgc cttccgctcg aagcggaaca
      181 ttccctacga ggacacgtgt ccggcggaag aggtgatccc gctgatcgac gccatctcgg
      241 acgtggccaa ggcgaagagc gacggccgcg gggtgcagat cgcggtgctg gagtacttcc
      301 tgaagaagaa gaccggctgc atctggggcg tgatcatctc gctgccgggc caagagaccg
      361 actgggagat ccccgctctg taccgcggca agaccacgca ccacgcccag ttctgcaacc
      421 acgtgcacaa cgaccagttc tacagcgtgt ttcatgatgg actgcg
//