LOCUS EY192290 386 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa44f03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY192290 VERSION EY192290.1 GI:159496820 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 386) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 928 was extended to value 929 (,) This trace has been recalled with phred original value before phred recall for SL was 153 original value before phred recall for SR was 585 PolyA tail found inside read...SR being truncated to 538 Potential full insert read...vec-to-vec length: 517 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..386 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtc gcgatgagtt ggaggacgag cgcgaagcaa acatcaaccc ggaaatattg 61 ccaatttccc tggagattcg aattcaaagg cgcccactct ggtcgagcgc tccgtgctgg 121 tcgacagacg cgcctcccga tctgggagca cctctgcaag tcgggcactc gccaatccca 181 tattcgttca aacgatgtgg gccgcgtcta aaatcctgtc cggacggaat gcttccggcg 241 agagtgtcta cctcaaaatg gactcgaaaa aggaattggt gacaagagaa gaagagaatt 301 tttggttctt gtgcacgaag cgctgcttat gtgatttcca agtctcattt tccatcccaa 361 tttccataat aaaaatgtaa ctttga //