LOCUS EY192287 449 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa44e05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_498265.1| Aspartyl aminopeptidase (51.2 kD) [Caenorhabditis elegans] sp|Q19087|DNPE_CAEEL Putative aspartyl aminopeptidase pir|T15946 hypothetical protein F01F1.9 - Caenorhabditis elegans gb|AAC46643.1| Hypothetical protein F01F1.9 [Caenor, mRNA sequence. ACCESSION EY192287 VERSION EY192287.1 GI:159496817 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 449) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 153 original value before phred recall for SR was 981 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..449 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggat ccaaacatca gattggtggc atgttacgac aatgaggagg tgggcagcga 61 tagtgcacaa ggggcgggat cccactttac ggggtggctg atgcgtcgta ttcttgcatg 121 cggagatcac cccaccgact ttgagtgcgc cattggtcgt tcttacctaa tctctgctga 181 ccaggcacac gcctgtcacc tgaattacgc gcaaaaacac gaggatcagc acaaaccgac 241 gttccatgga ggtgtcgtcg tcaaagtcaa cgtcaatcag cgatacgcca ccaccgcaat 301 tacccattct tttctgaagg tgatagctga tgctgcccga gttcctctac agaaagtggt 361 tgtcagacac gatagcccct gcggctcaac ggttggcccg attttggcgt ccaaattggg 421 attgcagacc atcgatgttg gctgtcccc //