LOCUS EY192285 345 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa44e02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY192285 VERSION EY192285.1 GI:159496815 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 345) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 927 was extended to value 927 (,) This trace has been recalled with phred original value before phred recall for SL was 153 original value before phred recall for SR was 609 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..345 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggaa aacgcgtccg aggagaacaa ggagaatttc ctggagcgga tgggcaacag 61 cagggagagc atcgataaat tgctgactct ctaccgggag gcgtgcaccg aacagtttgt 121 ggacagcgcc attttggacc cgaacgagag cgcactcacc ccgtccagca ctccaaagaa 181 tcaaactggc ggagcacagt tgtacttcaa cacggcggag tgaagatgtg tttggaccac 241 cctggtctgg gtgttctgct gcgatttgct tgccaattgg atgcctttta tgtgtattaa 301 tctatttaca actcctttgc tttcgttttg ctggatctct tgacc //