LOCUS       EY192283                 465 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa44d03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY192283
VERSION     EY192283.1  GI:159496785
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 726 was extended to value 727 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 651
            PolyA tail found inside read...SR being truncated to 618
            Potential full insert read...vec-to-vec length: 601
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 cggcggggaa tcttacaata aataacccat ggttctggga tcaccggaaa cattaccctc
       61 ccggcattta ttccaaaagg gaaaaaatct gggttgggaa tccttttctt tggaaacttc
      121 ctcaagtggt ttctcatttt ttggttcaac cccttttggt accaagcttt tctgttcatg
      181 gtgaatcgta acggcagtgc tgttgagacc actcctttgc tcggctcgcc tagagacaac
      241 aatcgctttc aagtgccgca accaaatgca tactaattga ttcaagaacc ttttcactat
      301 ttattcggca ttattaccat tcaaggaatt cgcataatca tttcttttaa atagaattgt
      361 gtaatctctc tgctccaccc tctttattcc agttggcctt atctcctctc ttgtctcttt
      421 tacgcttccg agttccgatt cgattaatag taaaactggt tttga
//