LOCUS EY192281 458 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa44c04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY192281 VERSION EY192281.1 GI:159496783 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 458) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 152 original value before phred recall for SR was 956 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..458 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggggc ggaagaggtg acttccaaat taatccacct acgaggcgac aatttgtctt 61 ccgaaccttt atttatcaaa ttcatcgaga gctcgatcca ctatgtgctt gtcagccgta 121 ccgtcgactt cctcgagcgg cgcatgttca acttttcaga cccccagctc caatggaaat 181 ggattcgcgc aacgccgaac ttcgcacaaa tttccctaaa tttcactaac aggggattcg 241 aaaacctggg aaaattttcc aatctcattc gcataagcgg atcgcagatc actcttatca 301 acaaggaatc gcagcatttg gagtgccaga gagacaccga tttgctacag aacgcacttc 361 tcctcttgac cgcccacttt cttctcaata ccacccaatt gcttgccaga gcctatggtt 421 ggcagacatt ccacgcaaac agcaatgcct tcgacacc //