LOCUS       EY192253                 514 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa38h07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_741074.1| Putative membrane protein, with a
            transmembrane domain, 2 coiled coil-4 domains, of bilaterial origin
            (60.0 kD) [Caenorhabditis elegans] gb|AAF59596.3| Hypothetical
            protein Y55B1BM.1a [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY192253
VERSION     EY192253.1  GI:159496699
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 561 (,)
            original QR value of 894 was extended to value 895 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 153
            original value before phred recall for SR was 714
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat tttgaaggcg tgcagaacaa gctcgaagaa gaaagcctca agagaaattc
       61 aaccaacgat gatgtcgaaa gcatagaaat gttgcgtaac caattacgcg agttggaaaa
      121 tgagcgtcga atattggagg caaatggagt cgacggacta ccattcgcgt tgcaacattt
      181 gcttcggcgt acatatgaga gtgaatgggg ttatttattc cagcagaagt tggagtgtct
      241 gcatgagatg cgcgaggcga aggaattggt agacaaaatg cgaaggaagc acaccagcat
      301 tatgaactcc ctcaaattgg ctactggggc tgcctcaggc acagacgaca ttgacaacaa
      361 gatattcgga ctaaaagcca gaatggagaa gatcaaactg gccctcaacg agtgtactca
      421 gagatgggct gaaatcgagt cgcattgtgg atttttccat aaccgattct tttggcgccg
      481 ttcaaacctt gaatgccagc ggtcttgccg ctaa
//